Human Neurotrophin-3

Catalog #: 90225-A
Size: 2 µg
$95
Species: Human
Host Species: E. coli
MW: 14 kDa
Genbank #: P20783
a.a: 139–257
Background:
NT-3 is found in neurons of the central nervous system. NT-3 is expressed also in muscles and its expression is down-regulated in denervated muscles. Many human gliomas express and secrete NT-3 into the conditioned medium. Some protein domains of NT-3 are identical with those of NGF and BDNF. NT-3 selectively supports the survival of neuronal cell populations, and prevents the death of cultured embryonic rat spinal motor neurons at picomolar concentrations. NT-3 has been shown to enhance sprouting of corticospinal tract during development and after adult spinal cord lesion. The activities of NT-3 and BDNF are additive in some systems. The biological activities of NT- 3 are mediated by a receptor belonging to the trk family of receptors with intrinsic tyrosine-specific protein kinase activity. NT-3 only binds weakly to the trk receptor which is a high-affinity receptor for NGF.
UniProt
P20783
Synonym(s):
NT3, Neurotrophin 3, NT-3, NGF-2, Nerve Growth Factor 2, NTF3
Purity:
≥97% by SDS-PAGE and HPLC
Endotoxin Level:
<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity:
The ED50 was determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 10-50 ng/ml.
Formulation:
Lyophilized from a 0.2 µm filtered PBS solution, pH 7.5.
Format:
lyophilized protein
Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Reference(s):
1. J. Immunol., Apr 2009, 182: 4705 - 4712.
2. J. Neurosci., Jan 2009, 29: 575 - 587.
3. J. Cell Biol., Dec 2006, 175: 1029 - 1042.
Warning(s):
Avoid freeze/thaw cycles
Amino Acid Sequence:
YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT
Scientific Category:
Cytokine/Growth Factors
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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