Human Glial growth factor (GGF2)

Catalog #: 90255-B
Size: 50 µg
Species: Human
Host Species: E. coli
MW: 34 kDa
Genbank #: Q02297
a.a: 51–365
Neuregulins (NDF, heregulin, GGF, ARIA, or SMDF) are EGF-like growth and differentiation factors that signal through tyrosine kinase receptors of the ErbB family. The ErbB2 and ErbB4 receptors cooperate in transmission of neuregulin-1 signals in the heart, whereas ErbB2 and ErbB3 cooperate in neural crest cells.
Recombinant human GGF2 (Glial growth factor) is a disulfide-linked monomeric protein consisting of 315 amino acid residues, and migrates as an approximately 34 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Neuregulin-1 isoform 9 (GGF2) was expressed in E. coli.
Glial growth factor 2, GGF2, neuregulin-1, NRG1, heregulin, acetylcholine receptor inducing activity, ARIA, GGF
≥95% by SDS-PAGE and HPLC
Endotoxin Level:
<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity:
The ED50 was determined by the dose-dependent phosphorylation of human MCF-7 cells was found to be <20 ng/ml, corresponding to a specific activity of 5 x 104 Units/mg.
Lyophilized from a 0.2 µm filtered 10 mM phosphate buffer, pH 7.0.
lyophilized protein
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

1. Marchionni MA, et al. Adv Exp Med Biol. 1999,468:283-95.
2. Dimayuga FO, et al. J Neuroimmunol. 2003 Mar,136(1-2):67-74.
Avoid freeze/thaw cycles
Amino Acid Sequence:
Scientific Category:
Cytokine/Growth Factors
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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