NET – CHOK1 Recombinant Cell Line

Catalog #

60557

$9,400 *

Size: 2 vials

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Recombinant CHOK1 cell line expressing human norepinephrine transporter (NET), Genbank accession number NM_001043.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

●

Synonyms

NET, norepinephrine transporter, CHO, NET Cell Line

●

Product Data Gallery

Western Blot of hNET expression in NET-CHO-K1 cells

Dose response of hNET activity in NET-CHO-K1 cells to reference inhibitor Duloxetine HCl. The IC50 of Duloxetine is ~ 33.3 nM

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1

Materials Required But Not Supplied

• Neurotransmitter Transporter Uptake Assay Kit (Molecular Devices #R6138)
• HBSS buffer (1×) (Hyclone #SH30588.01).
• Thaw Medium 3 (BPS Cat. #60186)
• Growth Medium 3B (BPS Cat. #79529)

UniProt #

Q7Z628

Mycoplasma Testing

The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.

Background

NET is a monoamine transporter responsible for the sodium-chloride (Na+/Cl−)-dependent reuptake of extracellular norepinephrine (NE), also known as noradrenaline. NET can also reuptake extracellular dopamine (DA). The reuptake of these two neurotransmitters is essential in regulating concentrations in the synaptic cleft. NETs, along with the other monoamine transporters, play a key role in depression and neurodegenerative diseases (NDD) such as Alzheimer's and Parkinson's disease. NET is the target of many antidepressants and recreational drugs. In addition, an overabundance of NET is associated with ADHD.

References

1. Zhou, J., Drugs Future. 2004; 29(12): 1235–1244.

2. Jorgensen, S., et al., Journal of Neuroscience methods. 2008; 169: 168-176.

Storage/Stability

Immediately upon receipt, store in liquid nitrogen.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3 (BPS Cat. #60186): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 3B (BPS Cat. #79529): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 500 μg/ml of Hygromycin B to ensure the recombinant expression is maintained.

Shipping Temperature

Dry Ice

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Scientific Category

Neuroscience

Regulatory Status

Research Use Only