Cathepsin B, His-tag

Catalog #: 80001
Size: 10 µg
$375
Species: Human
Host Species: FreeStyle 293-F cells
MW: 43 kDa
Genbank #: NM_001908
Tag(s): C-terminal His-tag
a.a: CD33 1–16, CathepsinB 18–339
Description:
Human cathepsin B (GenBank Accession No. NM_001908), CD33 signal peptide (a.a. 1-16) + CathepsinB (a.a. 18-339), with C-terminal His-tag, MW=43 kDa, expressed in FreeStyle 293-F cells.
UniProt
P07858
Synonym(s):
Amyloid Precursor Protein Secretase, CTSB, APP Secretase
Specific Activity:
≥ 4048 pmol/min/µg
Assay Conditions:
25 mM MES, pH 5.0, 10 µM substrate (Z-Leu-Arg-AMC), 10 min, room temperature. Prior to assay enzyme was activated with preincubation at 10 µg/ml in 25 mM MES, pH 5.0, 5 mM DTT for 15 min at RT.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Tu, C. et al., Cancer Res. 2008 Nov 15;68(22):9147-56.
2. Hwang, JH. et al., Cancer Lett. 2009 Jan 18;273(2):266-72.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Protease
Product Type:
Recombinant Protein
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