Human Brain-Derived Neurotrophic Factor

Catalog #: 90110-B
Size: 10 µg
$200
Species: Human
Host Species: E. coli
MW: 14 kDa
Genbank #: P23560
a.a: 129–247
Background:
BDNF is found in neurons of the central nervous system. It is expressed predominantly in hippocampus, cortex, and synapses of the basal forebrain. The biological activity of BDNF is mediated by a receptor that belongs to the trk family of receptors encoding a tyrosine-specific protein kinase. BDNF only binds weakly to the gp140trk receptor (to which NGF binds with high affinity), and it binds to the NGF receptor known as LNGFR. BDNF selectively supports the survival of primary sensory neurons and retinal ganglia. The factor supports survival and differentiation of certain cholinergic neurons and also some dopaminergic neurons in vitro. BDNF does not appear to act on sympathetic ganglia. In specific neurons of the central nervous system located in the hippocampus and the cortex the synthesis of BDNF is influenced by neuronal activity either positively (glutamate transmitter system) or negatively (GABA transmitter system).
Description:
Recombinant BDNF is a disulfide-linked homodimeric protein consisting of two 119 amino acid residues. migrates as an approximately 27 kDa protein under non-reducing conditions and as a 13-14 kDa protein under reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Brain Derived Neurotrophic Factor mature chain was expressed in E. coli.
UniProt
P23560
Synonym(s):
Brain-Derived Neurotrophic Factor, BDNF, Abrineurin
Purity:
≥97% by SDS-PAGE and HPLC
Endotoxin Level:
<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity:
The ED50 was determined by the dose-dependent proliferation of rat C6 cells, and was found to be in the range of 0.5 µg/ml.
Formulation:
Lyophilized from 0.2 µm filtered citric acid, pH 5.7.
Format:
lyophilized protein
Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Reference(s):
1. PNAS, Sep 2009, 106: 16481 - 16486.
2. J. Neurosci., Sep 2009, 29: 11650 - 11661.
Warning(s):
Avoid freeze/thaw cycles
Amino Acid Sequence:
HS DPARRGELSV CDSISEWVTA ADKKTAVDMS GGTVTVLEKV PVSKGQLKQY FYETKCNPMG YTKEGCRGID KRHWNSQCRT TQSYVRALTM DSKKRIGWRF IRIDTSCVCT LTIKRGR
Scientific Category:
Cytokine/Growth Factors
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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