NEK2 Recombinant

Catalog #

40009

$355 *

Size: 10 µg

Qty

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Description

Human NEK2 (GenBank Accession No. NM_002497) full length with N-terminal His-tag, MW=53 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 2 times.

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Synonyms

NEK2, NIMA-related kinase 2, Never In Mitosis-Related Kinase 2

Product Info

Storage and Usage

Citations2

Species

Human

Host Species/Expression System

Sf9 insect cells

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.05% Tween-20, 40% glycerol, and 3 mM DTT.

53 kDa

Amino Acids

full length

Specific Activity

100 U/mg

Unit Definition

One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of myelin basic protein (MBP) substrate per minute at pH 7.4 and 30°C.

Genbank #

NM_002497

UniProt #

P51955

Tag(s)

N-terminal His-tag

References

1. Fletcher, L. et al., Biochim. Biophys. Acta 1744(2):89-92 (2005).
2. Fardilha, M. et al., Ann. N.Y. Acad. Sci. 1030:468-478 (2004).

Storage/Stability

>6 months at -80°C.

Assay Conditions

50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP, 0.3 mg/ml MBP substrate, and 0.2 µg/ml NEK2.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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NEK2 Recombinant

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