NEDD8 E1, His-tag Recombinant
Catalog #
81087
$360
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Description
Human NEDD8 E1 recombinant protein complex, with His-tagged APPBP1 and untagged Uba3, co-expressed in E. coli.
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Synonyms
Nedd8, APPBP1/Uba3
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
E. coli
Purity
≥80%
Format
Aqueous buffer solution
Formulation
50 mM Tris Buffer, pH 8.0, 150 mM NaCl, 10% glycerol
MW
61 kDa (APPBP1), 52 kDa (UBA3)
Biological Activity
1–100 nM is used for the in vitro conjugation
UniProt #
Q8TBC4
Background
The NEDD8 activating E1 enzyme is a heterodimer composed of APPBP1 and UBA3 subunits. The APPBP1/UBA3 enzyme has homology to the N- and C-terminal halves of the ubiquitin E1 enzyme, respectively. The UBA3 subunit contains the catalytic center and activates NEDD8 in an ATP-dependent reaction by forming a high-energy thiolester intermediate. The activated NEDD8 is subsequently transferred to the UbcH12 E2 enzyme, and is then conjugated to specific substrates in the presence of the appropriate E3 ligases.
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