Stable recombinant HEK293 cell line expressing tetracycline-inducible human Nav1.8 (Genbank #NP_006505.2) fused to Green Fluorescent Protein (GFP) [Ex. ~395 nm, 475 nm; em ~510 nm] and the human sodium channel β2 subunit (Genbank #NM_004588; SCN2B). Nav1.8 is also known as tetrodotoxin-resistant voltage-gated sodium channel type X (SCN10A).
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Synonyms
NAV
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Product Data Gallery
Western blot of tetracycline-induced Nav1.8-GFP, probed with anti-GFP antibody
Western for β2 subunit-FLAG (constitutively expressed), probed with anti-FLAG antibody
Each vial contains ~1 X 106 cells in 10% DMSO solution
Amino Acid Sequence
A synthetic codon-optimized DNA sequence encoding human Nav1.8 (SCN10A gene) with C-terminal GFP-tag and C-terminal Streptavidin-Binding Peptide (SBP)-tag, and the SCN2B gene encoding human β2 subunit with C-terminal FLAG-tag are stably integrated in tetracycline-inducible HEK293 cells.
Genbank #
NP_006505.2, NM_004588
UniProt #
Q9Y5Y9, O60939
Mycoplasma Testing
The cell line is confirmed for absence of Mycoplasma species using the PCR-based VenorGeM® Mycoplasma Detection kit (Sigma Aldrich).
Background
Nav1.8 is a voltage gated type X, alpha subunit sodium channel which in humans is encoded by the SCN10A gene. It is expressed in nociceptors and has been proposed as a target for the development of new analgesics. Mice deficient in Nav1.8 have deficits in sensing inflammatory pain (initiated by tissue damage/inflammation) and visceral pain (initiated by damage or injury to internal organs) but not neuropathic pain. Native sodium channels are complexes composed of the pore-forming α subunit and an auxiliary β subunit. The sodium channel β2 subunit, encoded by the SCN2B gene, increases current amplitude and surface expression of sodium ion channels.
References
1. Catterall, W.A. et al. Pharmacol. Rev. 57 (4): 397–409 (2005). 2. Muzny, D.M., et al. Nature 440:1194-1198 (2006). 3. Wilson, D.S., et al. Protein Expression and Purification 23 (3): 440–446 (2001).
Storage/Stability
Immediately upon receipt, store in liquid nitrogen.
Growth Media
For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.
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