MYLIP/IDOL, His-FLAG-tag Recombinant

Catalog #
71208
$480 *
Size: 20 µg
Qty
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Description

Human MYLIP, also known as Inducible Degrader of the LDL receptor (IDOL), GenBank Accession No. NM_013262, a.a. 2-445(end), with N-terminal His-FLAG-tag, MW=51.7 kDa, expressed in a baculovirus expression system.

Synonyms
MYLIP, IDOL, Myosin Regulatory Light Chain Interacting Protein, Inducible Degrader Of The LDL-Receptor, BZF1,
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
Sf9 insect cells
Purity

≥89%

Format
Aqueous buffer solution
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, and 20% glycerol.
MW
51.7 kDa
Amino Acids
2–445(end)
Genbank #
NM_013262
UniProt #
Q8WY64
Tag(s)
N-terminal His-FLAG-tag
Background
MYLIP is an E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Its activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibition of neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
References
1. Santos PC., et al. Lipids Health Dis. 2012;11:83.
2. Sorrentino V., et al. Eur Heart J. 2013;34(17):1292-7.