Myc Reporter (Luc) – HCT116 Recombinant Cell Line (Myc Signaling Pathway)

Catalog #: 60520
Size: 2 vials
The Myc signaling pathway plays an important role in cell proliferation, differentiation, transformation and apoptosis. The c-Myc protein is a transcription factor that heterodimerizes with Max to regulate transcription of genes involved in proliferation, transformation and angiogenesis. Myc mutations have been linked to the development of a number of human cancers, including Burkitt’s lymphoma, cervical, ovarian, breast, lung and pancreatic carcinoma, making Myc a promising therapeutic target.
The Myc Reporter – HCT116 cell line contains the firefly luciferase gene under the control of Myc responsive elements stably integrated into HCT116 cells, a human colon cancer cell line. HCT116 contains a mutated beta-catenin which leads to the accumulation of β-catenin and constitutive activation of downstream Myc that induces the expression of Myc luciferase reporter. The cell line is validated for the inhibition of the expression of Myc luciferase reporter.
Materials Required But Not Provided:

ICG-001 (Selleckchem #S2662): an inhibitor of the Wnt/β-catenin pathway. Myc can be activated by the Wnt pathway.
Assay Medium 7B (BPS Bioscience #79718): Opti-MEM I (Life Technologies #31985-062) + 0.5% FBS + 1% Non-essential amino acids + 1 mM sodium pyruvate + 1% penicillin/streptomycin
96-well tissue culture plate or 96-well tissue culture-treated, white clear-bottom assay plate
ONE-Step™ Luciferase Assay System (BPS Cat. #60690). Other luciferase assay systems are also suitable.

Supplied As:
Each vial contains 1.5 X 106 cells in 1 ml of 10% DMSO.
Host Cell line:
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM® Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 7 (BPS Bioscience #60185): McCoy’s 5A medium (Hyclone #SH30200.01) with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone SV30010.01).

Growth Medium 7B (BPS Bioscience #79545): Thaw Medium 7 (BPS Cat. #60185) plus 400 µg/ml of Geneticin (Life Technologies #11811031).

Cells should be grown at 37°C with 5% CO2 using Growth Medium 7B.

Storage / Stability:
Immediately upon receipt, store in liquid nitrogen.
Monitor Myc pathway activity and ccreen for activators or inhibitors of the Myc pathway.

1. Pelengaris S, et al. (2002) c-MYC: more than just a matter of life and death. Nat. Rev. Cancer. 2(10): 764-76.

Application Reference(s):
1. Dynamic expression of tRNA-derived small RNAs define cellular states (2017)


License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Scientific Category:
Cell Signaling Pathway
Product Type:
Recombinant Cell Lines
Data shown is lot-specific. Contact us for specific information on other lots.

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