Myc Reporter Kit (Myc Signaling Pathway)

Catalog #: 60519
Size: 500 reactions
$525
Background:
The Myc signaling pathway plays an important role in cell proliferation, differentiation, transformation and apoptosis. The c-Myc protein is a transcription factor that heterodimerizes with Max to regulate Myc signaling pathway responsive genes. Myc mutations have been linked to the development of a number of human cancers, including Burkitt’s lymphoma, cervical, ovarian, breast, lung and pancreatic carcinoma, making Myc a promising therapeutic target for cancer treatment.
Description:
The Myc Pathway Reporter kit is designed for monitoring the activity of the Myc signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for c- Myc and Myc luciferase reporter vector. Inside the cells, c-Myc will bind to Max, translocate to the nucleus, and induce expression of the Myc luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized Myc responsive elements located upstream of a minimal promoter. The Myc reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency.

The kit also includes a non-inducible firefly luciferase vector premixed with constitutivelyexpressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity.

Format:
COMPONENTS

Instructions for use:
See data sheet for detailed transfection and assay protocols.
Storage / Stability:
Stable at least 12 months from date of receipt, when stored as directed (-20°C).
Application(s):
• Monitor Myc signaling pathway activity. • Screen activators or inhibitors of the Myc signaling pathway. • Study effects of RNAi or gene overexpression on the activity of the Myc pathway.
Reference(s):
1. Pelengaris S, et al. (2002) c-MYC: more than just a matter of life and death. Nat. Rev. Cancer. 2(10): 764-76.
Notes:
The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Cell Signalling Pathway
Product Type:
Reporter Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

60519.pdf