MuRF1, SUMO-His-tags Recombinant

Catalog #
81052
$395 *
Size: 25 µg
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Description

Human MuRF1 recombinant protein (Genbank Accession No. NP_115977), full length with N-terminal SUMO- and His-tags, expressed in E. coli.

Synonyms
TRIM63, tripartite motif-containing 63
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
E. coli
Purity
≥90% by SDS-PAGE
Format
Aqueous buffer solution
Formulation

50 mM Tris Buffer, pH 7.5, 150 mM NaCl, 10% glycerol, 5 mM DTT

MW
55 kDa
Amino Acids
full length
Biological Activity
Typical enzyme concentration of 100 nM - 5 mM is used for in vitro conjugation, depending on conditions.
Genbank #
NP_115977
UniProt #
Q969Q1
Tag(s)
N-terminal SUMO-His-tags
Background
MuRF1 (Muscle-specific RING-finger protein 1) is an E3 (ubiquitin protein ligase) that is involved in the conjugation of ubiquitin to target substrates along with E1 and E2 enzymes. MuRF1 is also known as TRIM63 (tripartite motif containing 63) containing a RING-finger/B-box/coiled-coil tripartite fold and is a member of the RING-domain E3 family. MuRF1 has been implicated as a component of atrophy-associated accelerated proteolysis. It is upregulated in a number of different models of atrophy and interacts with numerous myofibrillar proteins.
References
1. Bodine, S.C., et al., Identification of ubiquitin ligases required for skeletal muscle atrophy. Science , 2001. 294:1704-8.
2. Centner, T., et al., Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain. J Mol Biol. 2001. 306:717-26.
3. Kedar, V., et al., Muscle-specific RING finger 1 is a bona fide ubiquitin ligase that degrades cardiac troponin I. Proc Natl Acad Sci USA, 2004. 101:18135-40.
4. Mrosek, M., et al., Molecular determinants for the recruitment of the ubiquitin-ligase MuRF-1 onto M-line titin. FASEB J. 2007. 21:1383-92.