mTOR/Raptor/MLST8 Complex, FLAG-Tag, His-Tag (Human) Recombinant

Catalog #

40300

$465 *

Size: 10 µg

Qty

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Description

Complex of recombinant human mTOR (mechanistic target of rapamycin kinase), recombinant human MLST8 (mTOR associated protein, LST8 homolog), and recombinant human Raptor (regulatory associated protein of mTOR complex 1). The mTOR construct contains an N-terminal Flag-Tag encompassing amino acids 1362-2549(end). The MLST8 construct contains an N-terminal His-tag (6xHis), full length, encompassing amino acids 2-326(end). The Raptor construct contains an N-terminal His-tag (6xHis), full length encompassing amino acids 2-1335(end). Complex was co-expressed and affinity purified.

This product has been cited 11 times.

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Synonyms

mTOR, mechanistic target of rapamycin, FRAP, FKBP-Rapamycin Associated Protein, Mammalian Target Of Rapamycin, raptor, regulatory associated protein of mTOR, Mip1, MLST8, Mammalian Lethal With SEC13 Protein 8, GBL, Gable, POP3, LST8

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

mTOR/Raptor/mLST8 Complex Activity

Product Info

Storage and Usage

Citations11

Species

Human

Construct

mTOR (FLAG-1362-2549(end)) / Raptor (His-2-1335(end)) / MLST8 (His-2-326(end))

Host Species/Expression System

Sf9

Purity

≥55%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 80 ng/µl FLAG peptide

mTOR: 137 kDa; Raptor: 150 kDa; MLST8: 37 kDa

Amino Acids

mTOR: 1362-2549(end); Raptor: 2-1335(end); MLST8: 2-326(end)

Specific Activity

6.8 pmol/min/μg

Genbank #

mTOR: NM_004958; Raptor: NM_020761; MLST8: NM_022372

UniProt #

mTOR: P42345; MLST8: Q9BVC4; Raptor: Q8N122

Tag(s)

N-terminal FLAG-tag (mTOR), N-terminal His-tag (MLST8), N-terminal His-tag (Raptor)

References

1. Toral-Barza et al., (2005) Biochem. Biophys. Res. Commun. 24, 332(1):304-10.
2. Rubio-Viqueira and Hidalgo, M. (2006) Adv. Exp. Med. Biol. 587:309-327.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Activity was measured using ADP-Glo™ (Promega) reagents with 100 µM ATP, 0.1 mg/ml 4EBP1 and 5 mM MnCl₂ in 25 µl using kinase buffer (40 mM Tris HCl, pH 7.4, 20 mM MgCl₂, 0.1 mg/ml BSA, and 2 mM DTT). Reaction was incubated for 45 minutes at 30°C.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine, Immunotherapy

Regulatory Status

Research Use Only