MTAP, GST-tag Recombinant

Catalog #

50305

$515 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human MTAP (methylthioadenosine phosphorylase), GenBank Accession No. NM_002451, a.a. 2-end, with N-terminal GST-tag, MW=57 kDa, expressed in an E. coli expression system.

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Synonyms

methylthioadenosine phosphorylase, MTAP

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥80%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol, and 3 mM DTT

57 kDa

Amino Acids

2–end

Genbank #

NM_002451

UniProt #

Q13126

Tag(s)

N-terminal GST-tag

References

1. Fernandez-Irigoyen, J. et al., Biochem. J. 411 (2), 457-465 (2008)
2. Hellerbrand, C. et al., Carcinogenesis 27 (1), 64-72 (2006)

Storage/Stability

>6 months at –80°C

Assay Conditions

MTAP activity was measured using a xanthine oxidase-coupled method. MTA cleavage by MTAP produce adenine, which is then converted to 2,8- dihydroxyadenine by xanthine oxidase, leading to an increase in absorbance at 305 nm. 50 µl reaction mixtures contained MTAP, 50 mM KHsub>2PO₄, pH 7.4, 200 µM MTA, 0.02 units Xanthine Oxidase, and 1 mM DTT. Activity was measured at 37°C by continuous monitoring the absorbance at 305 nm for 30 min.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Metabolic Enzymes-Phosphorylase

Regulatory Status

Research Use Only

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Datasheets/Protocols

50305_Lot80902.pdf

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