Mouse CTLA4[Biotin]:B7-1 Inhibitor Screening Assay Kit

Catalog #

79515

$1,235 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Mouse CTLA4[biotin]:B7-1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of mouse CTLA4:B7-1 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled mouse CTLA4 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, B7-1 is coated on a 96-well plate. Next, CTLA4[biotin] is incubated with B7-1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against Mouse CTLA4[Biotin]:B7-1? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

activity assay kit, CTLA-4

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

This kit comes in a convenient 96-well format, with biotin-labeled mouse CTLA4, purified mouse B7-1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Rotating or rocker platform

Format

Catalog Number	Component	Amount	Storage
79001	Mouse CTLA4 (CD152), Fc-Biotin-labeled	2 µg	-80°C	Avoid freeze/ thaw cycles!
79058	Mouse B7-1 (CD86), Fc fusion	5 µg	-80°C
79742	Streptavidin-HRP	10 µl	+4°C
79311	3x Immuno Buffer 1	50 ml	-20°C
79728	Blocking Buffer 2	50 ml	+4°C
79670	ELISA ECL Substrate A	6 ml	Room Temp
79670	ELISA ECL Substrate B	6 ml	Room Temp
	White 96-well microplate	1	+4°C

Background

The activation of naïve T cells requires two signals, the specific T cell receptor recognition of MHC/Antigen on the surface of the antigen-presenting cell (APC), and the binding of B7-1 (CD80) ligand on the APC with the CD28 receptor on the T cell surface. Conversely, binding of CTLA4 to B7-1 on the T-cell surface results in an inhibitory signal and prevents T-cell activation. CTLA4:B7-1 interaction is an important drug target for the regulation of the host’s response to cancer.

References

1. Ohtani, H., et al., Lab Invest. 1997; 77(3): 231-241.

2. Rovert, C., et al., N. Engl. J. Med. 2011; 364: 2517-25262.

Storage/Stability

6 months from date of receipt when stored as directed.

Instructions for Use

See assay kit data sheet for detailed protocol

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only