CD137, Fc fusion (mIgG2a), Biotin-labeled (Mouse) HiP™ Recombinant

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Catalog #

71255

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Description

Mouse secreted CD137, also known as Tumor Necrosis Factor Receptor Superfamily member 9, TNFRSF9, and ILA, GenBank Accession No. NM_001561, a.a. 24-187, fused at the Cterminus to the Fc portion of mouse IgG2a, with a C-terminal AviTag^TM expressed in a HEK293 cell expression system. This protein is enzymatically biotinylated using AviTagTM technology. MW = 46.7 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

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Synonyms

Tumor Necrosis Factor Receptor Superfamily member 9, TNFRSF9, ILA, 4-1BB

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Gel Filtration Curve

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

HEK293

Purity

≥90%

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

46.7 kDa (monomer)

Amino Acids

24-187

Genbank #

NM_001561

UniProt #

P20334

Tag(s)

C-terminal Fc fusion (mIgG2a), AviTag™

References

1. Wilcox, R.A., et al., J. Clin. Invest. 2002; 109(5): 651-659.
2. Foell, J. et al., J. Clin. Invest. 2003; 111(10): 1505-1518.

Storage/Stability

At least 6 months at –80°C.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying protein binding and screening small molecules.

Shipping Temperature

dry ice

Notes

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to 100 μg/ml in PBS + glycerol and stored at -80°C.

Scientific Category

Immunotherapy/Cell Surface Receptor

Regulatory Status

Research Use Only

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Datasheets/Protocols

71255_Lot160309.pdf

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CD137, Fc fusion (mIgG2a), Biotin-labeled (Mouse) HiP™ Recombinant

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