PD-L1 (Monkey), Fc fusion (Human IgG1), Biotin-labeled HiP™ Recombinant

Catalog #

71154

$435 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Monkey secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274 and B7 homolog 1 (B7- H1), GenBank Accession No. XP_005581836.1, a.a. 25-167, fused at the C-terminus to the Fc portion of human IgG1 with an Avitag™. This is expressed in a HEK293 cell expression system and is enzymatically biotin-labeled using Avitag™ technology. MW = 54 kDa. Note: this protein runs at an apparent higher molecular weight due to glycosylation.

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Synonyms

Programmed Cell Death 1 Ligand 1, CD274, B7 homolog 1 (B7-H1)

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Inhibition of mkPD-L2 [B]: by Neutralizing PD-1 Ab

Gel Filtration Curve

Monkey PD-1: PD-L1[Biotinylated] inhibition curve using a chemiluminescent assay (#79863). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Macaca fascicularis

Host Species/Expression System

HEK293

Purity

≥80%

Format

Aqueous buffer solution

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

54 kDa

Amino Acids

25-167

Genbank #

XP_005581836.1

Tag(s)

C-terminal Fc fusion (Human IgG1) and biotin label

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

References

1. Freeman, G.J., et al., J. Exp. Med. 2000; 192(7):1027-34.
2. Dong, H., et al., Nat. Med. 1999; 5(12):1365-9.

Storage/Stability

At least 6 months at –80°C.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.

Shipping Temperature

-80°C

Notes

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

This protein runs at an apparent higher molecular weight due to glycosylation.

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to 100 μg/ml in PBS + glycerol and stored at -80°C.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

71154_Lot150529.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

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PD-L1 (Monkey), Fc fusion (Human IgG1), Biotin-labeled HiP™ Recombinant

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