Molecular Biology Services

Site-Directed Mutagenesis

BPS will carry out site-directed mutagenesis on the selected sequence to make specified base changes. The strategy may involve a PCR mutagenesis approach, or an oligonucleotide-directed approach, depending on the needs and specifics of the project.

All mutagenesis clones are screened by DNA sequence analysis to ensure that the desired base changes have been made, and to ensure sequence integrity of the remainder of the clone.

You will receive a mini-scale plasmid preparation, and a stab culture of the strain carrying the plasmid, along with a report documenting restriction analysis and DNA sequence analysis.

Please contact us for more information about our site-directed mutagenesis service.

Gene Cloning

Using a DNA sequence of your specification, BPS will design and supply primers for PCR amplification of the desired sequence from your DNA source (plasmid, or cDNA). The PCR product will be cloned directly into a T/A cloning vector or digested with restriction enzymes and subcloned into the plasmid vector of your choice.

All PCR products will be sequenced (both DNA strands) to ensure sequence integrity. You will receive a mid-scale plasmid preparation, and a stab culture of the strain carrying the plasmid, along with a report documenting restriction analysis and DNA sequence analysis.

Please contact us for more information about our gene cloning service.