MMP9 (Q279R), His-tag Recombinant

Catalog #

80215

$425 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Human matrix metallopeptidase-9 (MMP9), also known as gelatinase B, MANDP2, CLG4B, Macrophage gelatinase, GELB and EC 3.4.24, with Gln to Arg mutation on a.a. 279, GenBank Accession No. NM_004994), a.a. 20-707 (end) with C-terminal His-tag, MW=77.8 kDa, expressed in an HEK293 cell expression system.

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Synonyms

matrix metallopeptidase-9, MMP9, gelatinase B, MANDP2, CLG4B, Macrophage gelatinase, GELB, EC 3.4.24, MMP

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

MMP9 (Q279R) inhibition curve using a fluorogenic assay (#79915). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.

77.8 kDa

Amino Acids

20-707 (end)

Specific Activity

≥ 2500 pmole/min/µg

Genbank #

NM_004994

UniProt #

P14780

Tag(s)

C-terminal His-tag

References

1. Wenham, R.M., et al., J. Soc. Gynecol. Investig. 2003 Sep;10(6):381-7.
2. Li, X., et al., J. Cancer Res. Clin. Oncol. 2013 Sep;139(9):1433-47.

Storage/Stability

>6 months at -80°C

Assay Conditions

Activation: This is a proform enzyme and requires activation prior to testing activity. Dilute the enzyme to 100 ng/µl in a solution containing 50 mM HEPES, pH 7.4, 10 mM CaCl2, 0.05% Brij-35, and 1 mM APMA (amino-phenyl mercuric acetate). Incubate at 37ºC for 2 hours.
Assay conditions: Reaction mixture with 10 μM 390 MMP substrate 1 (Anaspec). Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc328/em393.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Protease

Regulatory Status

Research Use Only