MLL1/WDR5/Ash2L/RbBP5/DPY30 (MLL1/WARD Complex), FLAG, His-Tag Recombinant

Catalog #
51021
$670 *
Size: 50 µg
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Description

MLL1/WARD complex of human MLL1 (GenBank Accession No. NM_005933,) a.a. 3745-end with N-terminal His-tag and MW= 27 kDa, human WDR5 (GenBank Accession No. NM_017588), a.a. 22-end with N-terminal FLAG-tag and MW= 35 kDa, human Ash2L (GenBank Accession No. NM_001105214), a.a. 2-end with N-terminal His-tag and MW= 61 kDa, human RbBP5 (GenBank Accession No. NM_005057), a.a. 2-end with N-terminal His-tag and MW= 60 kDa, and human DPY30 (GenBank Accession No. NM_032574) a.a. 2-end with N-terminal His-tag and MW=12 kDa individually expressed in an E. coli expression system.

 

Synonyms
MLL, Myeloid/Lymphoid Or Mixed-Lineage Leukemia, HRX, ALL-1, CXXC7, WDSTS, KMT2A, TRX1, MLL1
Product Info
Storage and Usage
Citations
Species
Human
Construct
MLL1 (His-3745-3969(end)) / RbBP5 (His-2-538(end)) / Ash2L (His-2-534(end)) / WDR5 (FLAG-2-334(end)) / DPY30 (His-2-99(end))
Host Species/Expression System
E. coli
Purity

≥79%

Format
Aqueous buffer solution
Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, variable imidazole, 1 mM TCEP, 1 µM ZnCl2

MW
MLL1: 27 kDa; WDR5: 37 kDa; Ash2L: 61 kDa; RbBP5: 60 kDa; DPY30: 12 kDa
Amino Acids
MLL1: 3745-3969(end); RbBP5: 2-538(end); Ash2L: 2-534(end); WDR5: 2-334(end); DPY30: 2-99(end)
Genbank #
MLL1: NM_005933; WDR5: NM_017588; Ash2L: NM_001105214; RbBP5: NM_005057; DPY30: NM_032574
UniProt #
MLL1: Q03164; WDR5: P61964; Ash2L: Q9UBL3; RbBP5: Q15291; DPY30: Q9C005
Tag(s)
N-terminal FLAG-tag (WDR5), N-terminal His-tag (others)
Background
MLL is a histone methyltransferase that plays an essential role in early development and hematopoiesis. Part of a multiprotein complex that mediates both methylation of Lys4 of histone H3 (H3K4me) complex and acetylation of Lys16 of histone H4 (H4K16ac). Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias.
References
1. Patel, A., et al., J. Biol. Chem. 2009; 284(36): 24242-24256.
2. Mohan, M., et al., Nat. Rev. Cancer 2010; 10(10): 721-728.