MLL1/WDR5/Ash2L/RbBP5 (MLL1/WAR Complex), His-tag Recombinant

Catalog #

51022

$670 *

Size: 50 µg

Qty

Bulk/Custom

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Description

MLL1/WAR complex of human MLL1 (GenBank Accession No. NM_005933), a.a. 3745-end with N-terminal His-tag and MW= 27 kDa, human Ash2L (GenBank Accession No. NM_001105214), a.a. 2-end with N-terminal His-tag and MW= 61 kDa, and human RbBP5 (GenBank Accession No. NM_005057), a.a. 2-end with N-terminal His-tag and MW= 60 kDa, individually expressed in an E. coli expression system, and human WDR5 (GenBank Accession No. NM_017588), a.a. 2-end with N-terminal His-tag and MW= 37.4 kDa expressed in a baculovirus Sf9 expression system.

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Synonyms

MLL, Myeloid/Lymphoid Or Mixed-Lineage Leukemia, HRX, ALL-1, CXXC7, WDSTS, KMT2A, TRX1, MLL1;

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥82%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.4, 300 mM NaCl, 250 mM imidazole, 1 mM TCEP, 1 µM ZnCl₂, and 10% glycerol

183 kDa (complex)

Amino Acids

MLL 3745–end, other components 2–end

Genbank #

NM_005933 (MLL1), NM_017588 (WDR5), NM_001105214 (Ash2L), NM_005057 (RbBP5)

UniProt #

MLL1: Q03164; WDR5: P61964; Ash2L: Q9UBL; RbBP5: Q15291

Tag(s)

N-terminal His-tag (all)

Background

MLL is a histone methyltransferase that plays an essential role in early development and hematopoiesis. Part of a multiprotein complex that mediates both methylation of Lys4 of histone H3 (H3K4me) complex and acetylation of Lys16 of histone H4 (H4K16ac). Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias.

References

1. Patel, A., et al., J. Biol. Chem. 2009; 284(36): 24242-24256.
2. Mohan, M., et al., Nat. Rev. Cancer 2010; 10(10): 721-728.

Storage/Stability

>6 months at -80°C.

Assay Conditions

50 µl reaction mix (50 mM Tris, pH 8.8, 5 mM MgCl₂, 4 M DTT, 20 µM S-adenosylmethionine, and 5-50 ng MLL complex) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated K4 residue of histone H3, incubate 1 hr. Then, incubate 30 minutes with the secondary HRP-labeled antibody. Finally, add HRP chemiluminescent substrate and read luminescence.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only