METTL3/METTL14 Protein Complex, FLAG-Tag, His-Tag Recombinant

Catalog #

100105

$380 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Complex of Human Methyltransferase-like 3 (METTL3), GenBank Accession No. NM_019852, a.a. 2-580 (end) with N-terminal FLAG-tag, MW = 66 kDa and Methyltransferase-like 14 (METTL14), Genbank Accession No. NM_020961 a.a. 2-456 (end), with N-terminal His-tag, MW = 53 kDa, coexpressed by baculovirus in Sf9 insect cells.

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Synonyms

Human Methyltransferase-like 3, METTL3; Methyltransferase-like 14, RNA

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Product Data Gallery

4-20% SDS-PAGE Coomassie staining

Specific Activity

METTL3/METTL14 Complex inhibition curve using a chemiluminescent assay (#79614). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 Insect Cells

Purity

≥70%

Format

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3mM DTT, 100ug/ml flag peptide

METTL3: 66 kDa; METTL14: 53 kDa

Amino Acids

METTL3: 2-580; METTL14: 2-456

Genbank #

METTL3: NM_019852; METTL14: NM_020961

UniProt #

"METTL3: Q86U44; METTL14: Q9HCE5"

Tag(s)

METTL3: N-terminal FLAG-tag; METTL14: N-terminal His-tag

References

1. Liu J. et al. Nat. Chem. Biol. 2014. 10:93–95

2. Wang X. et al. Nature. 2016. 534:575-578

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Add 50μl reaction mix containing S-adenosylmethionine, METTL enzyme, and METTL buffer to wells coated with the substrate. Incubate overnight at room temperature. Add antibody against methylated adenosine, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only