Methyltransferase(s)

 

Methyltransferases are enzymes that transfer a methyl group from S-adenosylmethionine (SAM) to their substrates.DNA methyltransferases modify DNA by adding a methyl group to cytosines. DNA methylation of CpG dinucleotides suppresses gene expression and is critical for transcriptional silencing during imprinting, X-inactivation and other developmental processes. DNA methylation also plays a crucial role in the development of nearly all types of cancer.

Methyltransferases can also modify proteins; histone methyltransferases methylate lysines and arginines on histone side chains. Lysines may be mono-, di-, or trimethylated and arginines may be mono-, symmetrically or asymmetrically dimethylated. Each of these different ‘flavors’ of methylation can result in different chromatin conformations and gene expression levels.

Several histone methyltransferases have been linked to diseases, in particular cancer. The lysine methyltransferase EZH2 is a prime example of this. EZH2 is a methyltransferase that is part of the Polycomb Repressive complex (PRC2). EZH2, along with EED, RbAP48, SUZ12 and AEBP2, form a 5-member complex that methylates Lysine 27 of Histone H3 (H3K27). Abnormal expression of EZH2 has been linked to breast, prostate, endometrial, and other human cancers. Several point mutations in EZH2 cause it to hypermethylate H3K27, and are strongly associated with non-Hodgkins lymphomas and leukemias. Not surprisingly, inhibition of EZH2 is a key target for anti-cancer drug discovery. To aid in discovery, BPS offers a number of EZH2 mutant enzymes.

Aside from purified enzymes, BPS offers assay kits, inhibitors/activators, and substrates. The substrates offered by BPS include purified full-length histone proteins, tetramers, octamers, nucleosomes, and a number of modified histones. This diverse portfolio provides researchers with optimal flexibility in their experiments.

References:

1.  Vire, E. et al., Nature. 2006; 439: 871-874.
2.  Lyko, F. et al., J. Nat. Canc. Inst. 2005; 97(20): 1498-1506. 

 

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Catalog#SizeProduct NamePrice
51000 50 µg G9a (EHMT2), GST-tag (E. coli-derived)
$360
51001 20 µg G9a (EHMT2), GST-tag (Sf9-derived)
$360
52001L 96 reactions G9a Chemiluminescent Assay Kit
$715
52051 384 reactions G9a Homogeneous Assay Kit
$1,070
52042 1 mg Histone H1, Full Length, His-tag
$465
52043 100 µg Histone H1, Full Length, His-tag
$75
52036 0.5 mg Histone H2a, Full Length, Biotin-labeled, His-tag
$465
52049 50 µg Histone H2a, Full Length, Biotin-labeled, His-tag
$75
52025 100 µg Histone H2a, Full Length, His-tag
$75
52021 1 mg Histone H2a, Full Length, His-tag
$465
52022 1 mg Histone H2b, Full Length, His-tag
$465
52026 100 µg Histone H2b, Full Length, His-tag
$75
79055-2 1 mg Histone H3
$455
79055-1 100 μg Histone H3
$70
52013 500 µg Histone H3 (2-58), GST-tag
$325
52101 100 µg Histone H3 (K27M), His-tag
$460
53030 96 reactions Histone H3 Dimethyl-K27 ELISA Detection Kit
$540
52080 100 µg Histone H3 K27C, Full Length, His-tag
$460
52081 100 µg Histone H3 K27cMe1, Full Length, His-tag
$440
52082 100 µg Histone H3 K27cMe2, Full Length, His-tag
$440
52010 160 nmole Histone H3 peptide (1-21)
$195
52011 80 nmole Histone H3 peptide (1-21), Biotin-labeled
$230
52016 160 nmole Histone H3 peptide (21-44)
$195
52017 80 nmole Histone H3 peptide (21-44), Biotin-labeled
$230
52020 40 nmole Histone H3 Peptide (21-44), Monomethyl K27, Biotin-labeled
$230

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