Methyltransferase(s)

 

Methyltransferases are enzymes that transfer a methyl group from S-adenosylmethionine (SAM) to their substrates.DNA methyltransferases modify DNA by adding a methyl group to cytosines. DNA methylation of CpG dinucleotides suppresses gene expression and is critical for transcriptional silencing during imprinting, X-inactivation and other developmental processes. DNA methylation also plays a crucial role in the development of nearly all types of cancer.

Methyltransferases can also modify proteins; histone methyltransferases methylate lysines and arginines on histone side chains. Lysines may be mono-, di-, or trimethylated and arginines may be mono-, symmetrically or asymmetrically dimethylated. Each of these different ‘flavors’ of methylation can result in different chromatin conformations and gene expression levels.

Several histone methyltransferases have been linked to diseases, in particular cancer. The lysine methyltransferase EZH2 is a prime example of this. EZH2 is a methyltransferase that is part of the Polycomb Repressive complex (PRC2). EZH2, along with EED, RbAP48, SUZ12 and AEBP2, form a 5-member complex that methylates Lysine 27 of Histone H3 (H3K27). Abnormal expression of EZH2 has been linked to breast, prostate, endometrial, and other human cancers. Several point mutations in EZH2 cause it to hypermethylate H3K27, and are strongly associated with non-Hodgkins lymphomas and leukemias. Not surprisingly, inhibition of EZH2 is a key target for anti-cancer drug discovery. To aid in discovery, BPS offers a number of EZH2 mutant enzymes.

Aside from purified enzymes, BPS offers assay kits, inhibitors/activators, and substrates. The substrates offered by BPS include purified full-length histone proteins, tetramers, octamers, nucleosomes, and a number of modified histones. This diverse portfolio provides researchers with optimal flexibility in their experiments.

References:

1.  Vire, E. et al., Nature. 2006; 439: 871-874.
2.  Lyko, F. et al., J. Nat. Canc. Inst. 2005; 97(20): 1498-1506. 

 

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Catalog#SizeProduct NamePrice
51035 20 µg EZH2 (F667I)/EED/SUZH12/RbAp48/AEBP2, FLAG-tag, His-tag
$385
51023 20 µg EZH2 (H689Y)/EED/SUZ12/RbAp48/AEBP2, FLAG-tag, His-tag
$385
50279 20 µg EZH2 (non-complexed), His-GST-tags
$475
51019 20 µg EZH2 (P132S)/EED/SUZ12/RbAp48/AEBP2, FLAG-tag, His-tag
$385
51033 20 µg EZH2 (R679C)/EED/SUZH12/RbAp48/AEBP2, FLAG-tag, His-tag
$385
51029 20 µg EZH2 (Y641C) /EED/SUZ12/RbAp48/AEBP2, His-tag
$385
51017 20 µg EZH2 (Y641F) /EED/SUZ12/RbAp48/AEBP2, His-tag
$385
79276 384 reactions EZH2 (Y641F) Chemiluminescent Assay Kit
$2,675
52075 96 reactions EZH2 (Y641F) Chemiluminescent Assay Kit
$1,495
51011 20 µg EZH2 (Y641H) /EED/SUZ12/RbAp48/AEBP2, His-tag
$385
51028 20 µg EZH2 (Y641N) /EED/SUZ12/RbAp48/AEBP2, His-tag, FLAG-tag
$385
52076 96 reactions EZH2 (Y641N) Chemiluminescent Assay Kit
$1,495
79277 384 reactions EZH2 (Y641N) Chemiluminescent Assay Kit
$2,675
52078 384 reactions EZH2 (Y641N) TR-FRET Assay Kit
$1,495
51013 20 µg EZH2 (Y641S) /EED/SUZ12/RbAp48/AEBP2, His-tag
$385
51003 50 µg EZH2 / EED / SUZ12, His-tag, FLAG-tag
$630
52085 384 reactions EZH2 Chemiluminescent Assay Kit
$2,675
52009L 96 reactions EZH2 Chemiluminescent Assay Kit
$1,470
52059 384 reactions EZH2 Homogeneous Assay Kit
$2,980
52680 384 reactions EZH2(Y641F) TR-FRET Assay Kit
$1,495
52066 384 reactions EZH2-EED Binding Assay Kit
$1,495
51002 20 µg EZH2/EED, His-tag, FLAG-tag
$480
51099 50 µg EZH2/EED/SUZ12/RbAp48, His-tag, FLAG-tag
$820
51004 50 µg EZH2/EED/SUZ12/RbAp48/AEBP2, FLAG-tag, His-tag
$840
51098 20 µg EZH2/SUZ12/RbAp48, His-GST-tags, His-tag
$465

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