Methyltransferase(s)

 

Methyltransferases are enzymes that transfer a methyl group from S-adenosylmethionine (SAM) to their substrates.DNA methyltransferases modify DNA by adding a methyl group to cytosines. DNA methylation of CpG dinucleotides suppresses gene expression and is critical for transcriptional silencing during imprinting, X-inactivation and other developmental processes. DNA methylation also plays a crucial role in the development of nearly all types of cancer.

Methyltransferases can also modify proteins; histone methyltransferases methylate lysines and arginines on histone side chains. Lysines may be mono-, di-, or trimethylated and arginines may be mono-, symmetrically or asymmetrically dimethylated. Each of these different ‘flavors’ of methylation can result in different chromatin conformations and gene expression levels.

Several histone methyltransferases have been linked to diseases, in particular cancer. The lysine methyltransferase EZH2 is a prime example of this. EZH2 is a methyltransferase that is part of the Polycomb Repressive complex (PRC2). EZH2, along with EED, RbAP48, SUZ12 and AEBP2, form a 5-member complex that methylates Lysine 27 of Histone H3 (H3K27). Abnormal expression of EZH2 has been linked to breast, prostate, endometrial, and other human cancers. Several point mutations in EZH2 cause it to hypermethylate H3K27, and are strongly associated with non-Hodgkins lymphomas and leukemias. Not surprisingly, inhibition of EZH2 is a key target for anti-cancer drug discovery. To aid in discovery, BPS offers a number of EZH2 mutant enzymes.

Aside from purified enzymes, BPS offers assay kits, inhibitors/activators, and substrates. The substrates offered by BPS include purified full-length histone proteins, tetramers, octamers, nucleosomes, and a number of modified histones. This diverse portfolio provides researchers with optimal flexibility in their experiments.

References:

1.  Vire, E. et al., Nature. 2006; 439: 871-874.
2.  Lyko, F. et al., J. Nat. Canc. Inst. 2005; 97(20): 1498-1506. 

 

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Catalog#SizeProduct NamePrice
52002L 96 reactions PRMT5 Chemiluminescent Assay Kit
$715
52052 384 reactions PRMT5 Homogeneous Assay Kit
$1,495
52058 384 reactions PRMT8 Homogeneous Assay Kit
$1,040
52060 96 reactions SETD2 Chemiluminescent Assay Kit
$695
51056 96 reactions SetDB1 Chemiluminescent Assay Kit
$675
52006L 96 reactions SUV39H1 Chemiluminescent Assay Kit
$695
53011 96 reactions SUV4-20H1 Chemiluminescent Assay Kit
$695
27617-1 5 mg 3-Deazaneplanocin (DZNep)
$435
27617-2 10 mg 3-Deazaneplanocin (DZNep)
$605
27618-1 5 mg 3-Deazaneplanocin A (DZNep) hydrochloride
$435
27618-2 10 mg 3-Deazaneplanocin A (DZNep) hydrochloride
$605
52120 250 µL 400 µM S-Adenosylmethionine
$200
52200 30 ml 4x DNMT Assay Buffer 1
$195
52201 30 ml 4x DNMT Assay Buffer 2
$195
52160 30 ml 4x HMT Assay Buffer 1
$195
52160-A 30 ml 4x HMT Assay Buffer 1
$195
52170 30 ml 4x HMT Assay Buffer 2
$195
52170-A 30 ml 4x HMT Assay Buffer 2A
$195
52180 30 ml 4x HMT Assay Buffer 3
$195
52190 30 ml 4x HMT Assay Buffer 4
$195
52191 30 ml 4x HMT Assay Buffer 5
$195
52192 30 ml 4x HMT Assay Buffer 6
$195
52193 30 ml 4x HMT Assay Buffer 7
$195
50281 50 µg AEBP2, His-tag
$430
27307 25 mg AMI-1 (sodium salt)
$335

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