SUV4-20H1
Human, recombinant, N-terminal GST tag

Catalog #: 51090
Size: 50 µg

Description:
Human SUV4-20H1 (GenBank Accession
No. NM_017635), amino acids 2-387, with
N-terminal GST tag, MW= 70 kDa,
expressed in E.coli expression system.

Synonyms: KMT5B

Specific Activity: 0.0001 pmol/min/μg
Assay Conditions: 50 μl reaction mix (50
mM TRIS pH 8.8, 5mM MgCl2, 4mM DTT,
0.05% Tween-20, 800 μM S-adenosyl
methionine, and SUV4-20H1 add
to the wells coated with the substrate.
Incubate for 4 hr. Add primary
antibody 18, incubate 1 hr. Then, add
secondary HRP-labeled antibody and
incubate 30 min. Finally, add HRP
chemiluminescent substrates and read
luminescence.

Application:
Useful for the study of enzyme kinetics,
screening inhibitors, and selectivity
profiling.

Formulated in: 40 mM Tris-HCl, pH 8.0,
110 mM NaCl, 2.2 mM KCl,16 mM Glutathione,
20% Glycerol, and 3 mM DTT.

Stability: >6 months at –80°C

References:
1. Yang H. et al., 2008 J Biol Chem. 2008 May
2;283(18):12085-92.
2. Benetti R et al., J Cell Biol. 2007 Sep
10;178(6):925-36.

PRODUCT DATA SHEETS
Lot 130403-10

Data shown is lot-specific. For specific information on other lots, please contact us.
Related: HMT, Histone Methyltransferase, HKMT, Lysine