EZH2 (non-complexed), His-GST-tags

Catalog #: 50279
Size: 20 µg
$460
Species: Human
Host Species: Sf9 insect cells
MW: 114 kDa
Genbank #: NM_004456
Tag(s): N-terminal His-GST-tags
a.a: 2–end
Background:
EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
Description:
Human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end with N-terminal His-GST-tag, MW= 114 kDa, expressed in a Sf9 baculovirus expression system. This protein is inactive alone, although it is functional if combined with other proteins in the complex.
UniProt
Q15910
Synonym(s):
KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, EZH, KMT6
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

≥ 6 months at -80°C

Application(s):
Useful for Western blot analysis and to investigate protein-protein interactions in the EZH2 complex.
Reference(s):

1. Varambally, S. et al. Science 322 (5908), 1695-1699 (2008).
2. Rakotobe, D. et al., Virol. J. 5, 32 (2008).

Application Reference(s):
1. EZH2 contributes to the response to PARP inhibitors through its PARP-mediated poly-ADP ribosylation in breast cancer (2017)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Methyltransferase
Product Type:
Recombinant Protein
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Product Datasheets

50279_Lot140421-G4.pdf