EZH2 (F667I)/EED/SUZH12/RbAp48/AEBP2, FLAG-tag, His-tag

Catalog #: 51035
Size: 20 µg
$375
Species: Human
Host Species: Sf9 insect cells
MW: 325 kDa (complex)
Genbank #: NM_004456 (EZH2), NM_003797(EED), NM_015355 (SUZ12), NM_153207(AEBP2), NM_005610(RbAP48)
Tag(s): N-terminal FLAG (EED), N-terminal His-tag (rest)
a.a: 2–end (all components)
Background:
EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
Description:
Mutant version of BPS’s regular EZH2 5-member complex (Cat. # 51004), but with a Phe-to-Ile mutation on a.a. 672 of the EZH2 protein.* Complex of human EZH2 (GenBank Accession No. NM_004456), (a.a. 2-end; F672I) with N-terminal His-tag, MW=86 kDa, human EED (NM_003797) (a.a. 2-end) with N-terminal FLAG-tag, MW= 51 kDa, human SUZ12 (NM_015355) (a.a. 2-end) with N-terminal His-tag, MW = 87 kDa, Human AEBP2 (NM_153207) (a.a. 2-end) with N-terminal His-tag, MW= 53 kDa, and human RbAp48 (NM_005610) (a.a. 2-end) with N-terminal His-tag, MW = 48 kDa, co-expressed in a baculovirus expression system.
UniProt
Q15910
Synonym(s):
KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, RBAP48, Retinoblastoma Binding Protein 4 (RBBP4), hEED, Suppressor Of Zeste 12 Homolog, CHET9, JJAZ1; AE binding protein 2, Adipocyte Enhancer binding protein 2, EZH
Supplied As:
aqueous buffer solution
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol
Storage / Stability:

>6 months at -70°C.

Application(s):
Useful for Western blot analysis and to investigate protein-protein interactions in the EZH2 complex.
Reference(s):

1. Varambally, S. et al. Science 322 (5908), 1695-1699 (2008).
2. Rakotobe, D. et al., Virol. J. 5, 32 (2008).

Application Reference: Morin, R.D., et al. Nat Genet. 2010 February, 42(2): 181–185.

Notes:
*This mutation is equivalent to F667I in variant 3 (GenBank Accession No. NM_001203247).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Methyltransferase
Product Type:
Recombinant Protein
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Product Datasheets

51035_Lot120514.pdf