EZH2 A687V/EED/SUZ12/RbAp48/AEBP2
Human, recombinant

Catalog #: 51038
Size: 20 µg

Description: Mutant version of BPS
regular EZH2 5-member complex (Cat. #
51004), but with a Ala-to-Val mutation on
a.a. 692 of the EZH2 protein*. Complex of
human EZH2 (GenBank Accession No.
NM_004456), (a-a 2-end ; A692V) with N-
terminal His tag, MW= 86 kDa, human
EED (NM_003797) (a-a 2-end) with N-
terminal Flag tag, MW= 51 kDa, human
SUZ12 (NM_015355) (a-a 2-end) with N-
terminal His tag, MW = 87 kDa, Human
AEBP2 (NM_153207) (a-a 2-end) with N-
terminal His tag, MW= 53 kDa, and human
RbAp48 (NM_005610) (a-a 2-end) with N-
terminal His tag, MW = 48 kDa,
coexpressed in baculovirus expression
system. *This mutation is equivalent to
A687V in variant 3 (GenBank Accession
No. NM_001203247).

Application: Useful for the study of
enzyme kinetics, screening inhibitors, and
selectivity profiling.

Formulated in: 40 mM Tris-HCl, pH
8.0, 110 mM NaCl, 2.2 mM KCl, 20 mM
imidazole, and 20% glycerol.

Stability: >6 months at –80°C

References:
1. Varambally, S. et al. Science 322
(5908), 1695-1699 (2008).
2. Rakotobe, D. et al., Virol. J. 5, 32
(2008).

Application Reference:
Morin, R.D., et al. Nat Genet. 2010
February; 42(2): 181–185.

PRODUCT DATA SHEETS
Lot 120622

Data shown is lot-specific. For specific information on other lots, please contact us.
Related: HMT, Histone Methyltransferase, HKMT, Lysine