EZH1/EED/SUZ12, His-tag

Catalog #: 51006
Size: 50 µg
$610
Species: Human
Host Species: Sf9 insect cells
MW: 224 kDa (complex)
Genbank #: NM_001991 (EZH1), NM_003797(EED), NM_015355 (SUZ12)
Tag(s): N-terminal His-tag
a.a: 2–end (all components)
Description:
Complex of human EZH1, GenBank Accession No. NM_001991, a.a. 2-end, with N-terminal His-tag, MW= 86 kDa; human EED, NM_003797, a.a. 2-end, with N-terminal FLAG-tag, MW= 51 kDa; human SUZ12, NM_015355, a.a. 2-end, with N-terminal His-tag, MW = 87 kDa, co-expressed in a baculovirus expression system.
UniProt
Q15910
Synonym(s):
KMT6B, EZH
Specific Activity:
≥0.02 pmol/min/µg.
Assay Conditions:
Enzyme was incubated with 2 µM histone mixture with 40 µM AdoMet in HMT buffer 2A in a 10 µl reaction for 60 min at RT. 5 µl of Anti-Rabbit AlphaLISA® acceptor beads (1:250) and 5 µl of Primary antibody 6 (1:50) were added and incubation continued for 30 min. Nickel Donor beads (1:125) were added (10 µl) and mixture was further incubated for 45-60 min at RT before A-counts were measured.
Formulation:
40 mM Tris-HCl, pH 8.0, 10 mM NaCl, 2.2 mM KCl, 80 ng/µl FLAG peptide, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Margueron R. et al. Mol. Cell 32, 4, 503-518, 2008.
2. Shen X. et al., Mol. Cell 32, 4, 491-502, 2008.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Methyltransferase
Product Type:
Recombinant Protein
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Product Datasheets

51006_Lot131206.pdf