EHMT1 (GLP), GST-tag

Catalog #: 51020
Size: 50 µg
Species: Human
Host Species: E. coli
MW: 72.4 kDa
Genbank #: NM_024757
Tag(s): N-terminal GST-tag
a.a: 894–1298 (end)
Human euchromatic histone-lysine N-methyltransferase (EHMT1), also known as G9a-like protein, GLP or KMT1D, GenBank Accession No. NM_024757, a.a. 894-1298 (end) with N-terminal GST-tag, MW= 72.4 kDa, expressed in an E. coli expression system.
EHMT1, GLP, KMT1D, G9a-like protein, euchromatic histone-lysine N-methyltransferase
Specific Activity:
≥122.3 pmole/min/µg
Assay Conditions:
50 μl reaction mix (50 mM Tris pH 8.8, 5 mM MgCl2, 4 mM DTT, 0.05% Tween-20, 40 μM S-adenosylmethionine, and EHMT1 (0.05 – 0.5 ng)) is added to substrate-coated wells on a Neutravidin black plate (G-Biosciences). Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol, and 3 mM DTT
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
1. Huang, J., et al., J Biol. Chem. 2010 Mar 26; 285(13): 9636-41.
2. Ueda, J., et al., J Biol. Chem. 2006 Jul 21; 281(29): 20120-8.
Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets