DNMT3A/DNMT3L Complex, His-tag, GST-tag

Catalog #: 51106
Size: 10 µg
$460
Species: Human
Host Species: Sf9 insect cells
MW: 86 kDa (complex)
Genbank #: NM_175629(DNMT3A), NM_013369 (DNMT3L)
Tag(s): N-terminal His, N-terminal GST-tag
a.a: DNMT3A: 623-912 (end), DNMT3AL: 160-387(end)
Description:
Complex of active human DNA methyl-transferase 3A (DNMT3A), GenBank Accession No. NM_175629, a.a 623-912(end) with N-terminal His-tag, MW=34 kDa, and DNMT3L (GenBank Accession No. NM_013369), a.a. 160-387(end), with an N-terminal GST-tag, MW = 52 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system.
UniProt
Q9Y6K1 (DNMT3A), Q9UJW3 (DNMT3L)
Specific Activity:
≥0.58 pmol/min/µg.
Assay Conditions:
Assayed using BPS Bioscience DNMT3A Assay Kit, Cat. #52033. Add 50 µl reaction mix containing DNMT3A/DNMT3L to wells coated with DNMT substrate. Incubate 2 hr at 37°C. Add antibody against 5-methylcytosine, then HRP-labeled secondary antibody followed by chemiluminescent detection.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 16 mM glutathione and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at –80°C.

Application(s):
Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Methyltransferase
Product Type:
Recombinant Protein
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Product Datasheets

51106_Lot100824.pdf