DNMT1, His-FLAG-tag Recombinant

Catalog #

51100

$455 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Recombinant human DNMT1 (DNA methyltransferase 1, isoform b), encompassing amino acids 501-1632 (end). This construct contains an N-terminal His-tag (6xHis) followed by a FLAG tag. The protein was affinity purified.

This product has been cited 8 times.

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Synonyms

TRDMT1, DNA, DNA (cytosine-5)-methyltransferase 1, DNMT1, ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI, DNA (cytosine-5-)-methyltransferase 1, DNMT-1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

DNMT1 Activity

Product Info

Storage and Usage

Citations8

Species

Human

Construct

DNMT1 (His-FLAG-501-1632(end))

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/µl FLAG peptide, and 20% glycerol

130 kDa

Amino Acids

501–1632 (end)

Genbank #

NM_001130823

UniProt #

P26358-2

Tag(s)

N-terminal His-FLAG-tag

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was performed using the DNMT1 Chemiluminescent Assay Kit (BPS Bioscience #52050L) with DNMT1 titrated from 800 ng/rxn – 12.5 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only