The SUV4-20H1 Chemiluminescent Assay Kit is designed to measure
SUV4-20H1 activity for screening and profiling applications. The key to the SUV4-20H1
Chemiluminescent Assay Kit is a highly specific antibody that recognizes a methylated
residue of Histone H4. With this kit, only three simple steps are required for
methyltransferase detection. First, S-adenosylmethionine is incubated with a sample
containing assay buffer and methyltransferase enzyme. Next, primary antibody is added.
Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition
of the HRP substrate to produce chemiluminescence that can be measured using a
chemiluminescence reader.
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Synonyms
Suppressor Of Variegation 4-20 Homolog 1 , KMT5B, SUV420H1
Kit comes in a convenient format, with 96-well plate precoated with histone H4 substrate, reagents, and purified SUV4-20H1 enzyme for 96 enzyme reactions.
Format
COMPONENTS
UniProt #
Q4FZB7
References
Dillon S.C., et al. 2005. Genome Biology 6:227.
Storage/Stability
6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay kit data sheet for detailed protocol.
Applications
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Shipping Temperature
-80°C
Contraindications
DMSO >1%, strong acids or bases, ionic detergents, high salt
Warnings
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
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