SUV39H2 Activity Assay Kit

Catalog #
52008L
$725 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The SUV39H2 Direct Activity Assay Kit is designed to measure SUV39H2 activity for profiling and screening applications, using purified SUV39H2. The SUV39H2 Direct Activity Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, the antibody against methylated lysine residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified SUV39H2 enzyme for 100 enzyme reactions. The key to the SUV39H2 Activity Assay Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate is added to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Synonyms
KMT1B, SUV39H2, Suppressor Of Variegation 3-9 Homolog 2 (Drosophila), Su(Var)3-9 Homolog 2 Inhibitor screening
Product Info
Storage and Usage
Citations
Assay Kit Format
Chemiluminescent
Supplied As
8-well strip plate precoated with histone substrate, Primary antibody methylated lysine residue of Histone H3, Secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified SUV39H2 enzymes for 100 enzyme reactions.
Format
Catalog # Component Amount Storage
51080 SUV39H2 4 µg -80°C Avoid freeze/ thaw cycles
52120 100 µM S-adenosylmethionine 250 µl -80°C
52140A Primary antibody 1 100 µl -80°C
52130H Secondary HRP-labeled antibody 1 10 µl -80°C
52160 4x HMT Assay Buffer 1* 3 ml -20°C
52100 Blocking buffer 4 50 ml +4°C
  HRP chemiluminescent substrate (2 components) 6 ml each +4°C
  96-well plate precoated with histone substrate 1 plate +4°C

*Add 125 µl of 0.5 M DTT before use.

UniProt #
Q9EQQ0
References
Dillon S.C., et al. 2005. Genome Biology 6:227.