PRMT9 Homogeneous Assay Kit

Catalog #

52069

$2,560 *

Size: 384 reactions

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The PRMT9 Homogeneous Assay Kit is designed to measure PRMT9 activity for screening and profiling applications. PRMT9 is a histone methyltransferase that exhibits methylation activity toward an arginine residue of SAP145. The key to the PRMT9 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT9 enzyme is incubated with the substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Need us to run inhibitor screens or profile your compounds against PRMT9? Check out our Methyltransferase Screening Services.

●

Synonyms

PRMT-9

●

Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

AlphaLISA®

Supplied As

The PRMT9 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated PRMT9 substrate, primary antibody, methylation assay buffer, and purified PRMT9 for 384 enzyme reactions.

Materials Required But Not Supplied

AlphaLISA® anti-rIgG acceptor beads, 5 mg/ml (PerkinElmer #AL104C)
AlphaScreen® Streptavidin-conjugated donor beads, 5 mg/ml (PerkinElmer #6760002S)
Optiplate-384 (PerkinElmer #6007290)

Format

Catalog Number	Component	Amount	Storage
79124	PRMT9*	2 x 50 µg	-80°C	Avoid freeze/ thaw cylces!
52120-A	250 µM S-adenosylmethionine	2 x 400 µl	-80°C
52140Z3	Primary antibody 28	20 µl	-80°C
	PRMT9 substrate	200 µl	-80°C
52170-B	4x HMT assay buffer 2B	3 ml	-20°C
	4x Detection Buffer 3	2 ml	-20°C
	Protective film (to seal the wells)

*The concentration of PRMT9 is lot-specific and will be indicated on the tube containing the enzyme.

UniProt #

Q6P2P2

References

Li Y., et al., J. Neurosci. 2015; 35(37): 12890-12902.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and HTS applications.

Shipping Temperature

-80°C

Notes

AlphaScreen^® and AlphaLISA^® are registered trademarks of PerkinElmer, Inc.

Contraindications

Green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen® assays.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

PRMT9 Homogeneous Assay Kit

PRODUCTS

SERVICES

RESOURCES

COMPANY