PRMT8 Homogeneous Assay Kit

Catalog #

52058

$1,125 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The PRMT8 Homogeneous Assay Kit is designed to measure the activity of PRMT8 for screening and profiling applications, using AlphaLISA^® technology. The key to the PRMT8 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT8 enzyme is incubated with the biotinylated substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Need us to run inhibitor screens or profile your compounds against PRMT8? Check out our Methyltransferase Screening Services.

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Synonyms

PRMT-8

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

AlphaLISA®

Supplied As

The PRMT8 Homogeneous Assay Kit comes in a convenient AlphaLISA^® format, with a 384-well plate, biotinylated histone H4 peptide substrate, primary antibody, methylation assay buffer, and purified PRMT8 for 384 enzyme reactions.

Materials Required But Not Supplied

AlphaLISA® anti-rIgG acceptor beads, 5 mg/ml (Perkin Elmer #AL104C)
AlphaScreen® Streptavidin-conjugated donor beads, 5 mg/ml (Perkin Elmer # 6760002)
OptiPlate-384 (Perkin Elmer #6007290)
AlphaScreen® microplate reader

Format

Catalog Number	Components	Amount	Storage
51052	PRMT8	50 µg	-80°C	*Avoid* *freeze/ thaw* *cycles!*
52120	100 µM S-adenoslymethionine	250 µl	-80°C
52150-3	Primary antibody 4-3	400 µl	-80°C
	Biotinylated histone H4 peptide substrate	400 µl	-80°C
52170-A	4x HMT Assay Buffer 2A	3 ml	-20°C
52301	4x Detection Buffer	2 ml	-20°C

UniProt #

Q9NR22

Background

PRMT8 is a histone methyltransferase that exhibits methylation activity toward histones H2A and H4.

References

1. Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1) :37-50.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Notes

AlphaScreen^® and AlphaLISA^® are registered trademarks of PerkinElmer, Inc.

Contraindications

Avoid green and blue dyes that absorb light in the Alphascreen^® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN₃) or metal ions (Fe²⁺, Fe³⁺, Cu²⁺, Zn²⁺ and Ni²⁺). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen^® assays.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

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PRMT8 Homogeneous Assay Kit

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