PRMT8 Chemiluminescent Assay Kit

Catalog #
52062
$750 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
Purchase
Description

The PRMT8 Chemiluminescent Assay Kit is designed to measure PRMT8 activity for screening and profiling applications. The PRMT8 Chemiluminescent Assay Kit comes in a convenient format with a 96-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine 3 (R3) residue of Histone H4, the secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase assay buffer, and purified PRMT8 enzyme for 100 enzyme reactions. The key to the PRMT8 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against PRMT8? Check out our Methyltransferase Screening Services

Synonyms
protein arginine N-methyltransferase 8, PRMT8, Histone-arginine N-methyltransferase 8, HRMT1L3, HRMT1L4, PRMT-8
Product Info
Storage and Usage
Citations
Assay Kit Format
Chemiluminescent
Materials Required But Not Supplied

TBST buffer (1x TBS, pH 8.0, containing 0.05% Tween-20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format

Catalog #

 Components

Amount

Storage

51052

PRMT8 His-FLAG-tags

10 µg

-80°C

Avoid freeze/
thaw cycles!

52120

20 μM S-adenosylmethionine*

250 µl

-80°C

52150

Primary antibody 4

100 µl

-80°C

52170

4x HMT assay buffer 2

3 ml

-80°C

52131H

Secondary HRP-labeled Antibody 2

10 µl

-80°C

52100

Blocking buffer 4

50 ml

+4°C


79670 

HRP chemiluminescent substrate A (translucent bottle)

6 ml

+4°C

HRP chemiluminescent substrate B (brown bottle)

6 ml 

+4°C

 

96-well plate precoated with histone substrate

1

+4°C

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

*Decreasing the S-adenosylmethionine concentration will make the assay more sensitive to test inhibitors.

UniProt #
Q9NR22
References

Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.