PRMT8 Chemiluminescent Assay Kit
The PRMT8 Chemiluminescent Assay Kit is designed to measure PRMT8 activity for screening and profiling applications. The PRMT8 Chemiluminescent Assay Kit comes in a convenient format with a 96-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine 3 (R3) residue of Histone H4, the secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase assay buffer, and purified PRMT8 enzyme for 100 enzyme reactions. The key to the PRMT8 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
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TBST buffer (1x TBS, pH 8.0, containing 0.05% Tween-20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform
Catalog # |
Components |
Amount |
Storage |
|
10 µg |
-80°C |
Avoid freeze/ |
||
250 µl |
-80°C |
|||
52150 |
Primary antibody 4 |
100 µl |
-80°C |
|
3 ml |
-80°C |
|||
10 µl |
-80°C |
|||
50 ml |
+4°C |
|||
6 ml |
+4°C |
|||
6 ml |
+4°C |
|||
96-well plate precoated with histone substrate |
1 |
+4°C |
*Decreasing the S-adenosylmethionine concentration will make the assay more sensitive to test inhibitors.
Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.