PRMT7 Homogeneous Assay Kit (Histone H2b)

Catalog #
79720
$1,125 *
Size: 384 Reactions
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*US Pricing only. For international pricing, please contact your local distributor.
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Description

The PRMT7 Homogeneous Assay Kit (Histone H2b) is designed to measure PRMT7 activity for screening and profiling applications. PRMT7 methylates arginine residues on various protein substrates and is involved in DNA transcription, RNA splicing, DNA repair, cell differentiation, and metastasis. The advantage of the PRMT7 Homogeneous Assay Kit is a highly specific antibody that specifically recognizes the methylated substrate. With this kit, only three steps are required for methyltransferase detection. First, a sample containing PRMT7 enzyme is incubated with the substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

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Synonyms
Protein Arginine Methyltransferase 7, [Myelin Basic Protein]-Arginine N-Methyltransferase PRMT, Histone-Arginine N-Methyltransferase PRMT7, Protein Arginine N-Methyltransferase 7, EC 2.1.1.321, KIAA1933, EC 2.1.1, SBIDDS, PRMT-7
Product Info
Storage and Usage
Citations
Assay Kit Format
AlphaLISA®
Supplied As
The PRMT7 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with His-tagged PRMT7 substrate, primary antibody, methylation assay buffer, and purified PRMT7 for 384 enzyme reactions.
Materials Required But Not Supplied
  • AlphaLISA® anti-rIgG acceptor beads, 5 mg/ml (PerkinElmer #AL104C)
  • AlphaScreen® Nickel donor beads, 5 mg/ml (PerkinElmer #AS101D)
  • Optiplate-384 (PerkinElmer #6007290)
  • Adjustable micropipettor and sterile tips 
  • Dithiothreitol (DTT), 0.5 M
Format
Catalog # Component Amount Storage
51054 PRMT7* 40 µg -80°C  
52120 20 μM S-adenosylmethionine 2 x 250 µl -80°C Avoid
freeze/
thaw
cycles!
52140Z3 Primary Antibody 28 20 µl -80°C
  PRMT7 Histone substrate 200 µl -80°C
  4x PRMT7 assay buffer
(add DTT before use)
3 x 1 ml -20°C
  4x Detection Buffer 3 2 ml -20°C

*The concentration of the proteins is lot-specific and will be indicated on the tube.

UniProt #
Q9NVM4
References
You Feng, et al. 2014. J Biol Chem 289:32604.