PRMT5 Chemiluminescent Assay Kit

Catalog #
52073
$1,630 *
Size: 384 reactions
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Description

The PRMT5 Chemiluminescent Assay Kit is designed to measure PRMT5 activity for screening and profiling applications. The key to the PRMT5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Synonyms
PRMT5, HRMT1L5, IBP72, JBP1, SKB1, PRMT-5
Product Info
Storage and Usage
Citations
Assay Kit Format
Chemiluminescent
Supplied As
The PRMT5 Chemiluminescent Assay Kit comes in a convenient format, with a 384-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine3 residue of Histone H4, the secondary HRP-labeled antibody, S-adenosylmethionine
Format
Catalog # Components Amount Storage
51045 PRMT5/MEP50 human enzyme 40 μg -80°C Avoid freeze/ thaw cycles!
52120 100 μM S-adenosylmethionine 250 μl -80°C
52150-3 Primary antibody 4-3 100 μl -80°C
52131H Secondary HRP-labeled antibody 2 2 x 10 μl -80°C
  4x PRMT5 assay buffer (add DTT
before experiment)
2 x 3 ml -20°C
52100 Blocking Buffer 4 2 x 50 ml +4°C
  HRP chemiluminescent substrate A (transparent bottle) 2 x 6 ml +4°C
  HRP chemiluminescent substrate B (brown bottle) 2 x 6 ml +4°C
  384-well plate precoated with histone substrate 1 +4°C
UniProt #
O14744
Background
PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3.
References
Stopa, N., et al. 2015. Cell Mol Life Sci. 72(11):2041-59.