PRMT3 Chemiluminescent Assay Kit

Catalog #

52005L

$750 *

Size: 96 reactions

Qty

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The PRMT3 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT3 activity for screening and profiling applications. The key to the PRMT3 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

This product has been cited 1 time.

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Synonyms

PRMT3, HRMT1L3, PRMT-3

Product Info

Storage and Usage

Citations1

Assay Kit Format

Chemiluminescent

Supplied As

Kit comes in a convenient format, with a 96-well plate precoated with specific substrate, reagents, and purified PRMT3 enzyme for 100 enzyme reactions.

Materials Required But Not Supplied

TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format

Catalog Number	Component	Amount	Storage
51043	PRMT3 human recombinant enzyme	10 µg	-80°C	Avoid freeze/ thaw cycles!
52120	52120 20 μM S-adenosylmethionine*	250 µl	-80°C
52150	Primary antibody 4	100 µl	-80°C
52131H	Secondary HRP-labeled antibody 2	10 µl	-80°C
52170	4x HMT assay buffer 2	3 ml	-20°C
52100	Blocking buffer 4	50 ml	+4°C
	HRP chemiluminescent A (transparent bottle)	6 ml	+4°C
	HRP chemiluminescent B (brown bottle)	6 ml	+4°C
	96-well plate precoated with histone substrate	1 plate	+4°C

*Decreasing S-adenosylmethionine concentration will make the assay more sensitive to inhibitors.

UniProt #

Q8WUV3

References

Dillon S.C., et al. 2005. Genome Biology 6:227.

Storage/Stability

6 months from date of receipt when stored as directed.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

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PRMT3 Chemiluminescent Assay Kit

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