PRMT1 Homogeneous Assay Kit

Catalog #: 52054
Size: 384 reactions
$1,040
Background:
PRMT1 is a histone methyltransferase that exhibits methylation activity toward H4-R3.
Description:
The PRMT1 Homogeneous Assay Kit is designed to measure the activity of PRMT1 for screening and profiling applications, using AlphaLISA® technology. The key to the PRMT1 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT1 enzyme is incubated with the biotinylated substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Synonym(s):
PRMT1, HRMT1L2, IR1B4, HCP1, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen
Supplied As:
The PRMT1 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone H4 peptide substrate, primary antibody, methylation assay buffer, and purified PRMT1 for 384 enzyme reactions.
Contraindications:
Avoid green and blue dyes that absorb light in the Alphascreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen® assays.
Format:
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):

Li Y., et al., J. Neurosci. 2015; 35(37): 12890-12902.

Application Reference(s):
Novel symmetrical ureas as modulators of protein arginine methyl transferases (2013)

Notes:
AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Methyltransferase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

52054.pdf