PRMT1 Homogeneous Assay Kit

Catalog #

52054

$1,180 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The PRMT1 Homogeneous Assay Kit is designed to measure PRMT1 activity for screening and profiling applications. PRMT1 is a histone methyltransferase that exhibits methylation activity toward H4-R3. The key to the PRMT1 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated histone substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT1 enzyme is incubated with the biotinylated substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alphacounts.

Need us to run inhibitor screens or profile your compounds against PRMT1? Check out our Methyltransferase Screening Services.

This product has been cited 2 times.

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Synonyms

PRMT1, HRMT1L2, IR1B4, HCP1, PRMT-1

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Product Data Gallery

Product Info

Storage and Usage

Citations2

Assay Kit Format

AlphaLISA®

Supplied As

The PRMT1 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with a 384-well plate, biotinylated histone H4 peptide substrate, primary antibody, methylation assay buffer, and purified PRMT1 for 384 enzyme reactions.

Materials Required But Not Supplied

AlphaLISA anti-rIgG acceptor beads, 5 mg/ml (Perkin Elmer #AL104C)
AlphaScreen Streptavidin-conjugated donor beads, 5 mg/ml (Perkin Elmer # 6760002)
OptiPlate-384 (Perkin Elmer #6007290)
AlphaScreen microplate reader

Format

Catalog #	Components	Amount	Storage
51040	PRMT1	1 µg	-80°C	Avoid freeze/ thaw cycles!
52120	20 μM S-adenosylmethionine	2 x 250 µl	-80°C
52150-3	Primary antibody 4-3	4 x 100 µl	-80°C
	Biotinylated histone H4 peptide substrate	1000 µl*	-80°C
52170-A	4x HMT assay Buffer 2A	3 ml	-20°C
	4x Detection Buffer	2 ml	-20°C

*Solubilize in 1,000 µl of distilled water.

UniProt #

Q99873

Background

PRMT1 is a histone methyltransferase that exhibits methylation activity toward H4-R3.

References

Li Y., et al., J. Neurosci. 2015; 35(37): 12890-12902.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Notes

AlphaScreen^® and AlphaLISA^® are registered trademarks of PerkinElmer, Inc.

Contraindications

Avoid green and blue dyes that absorb light in the Alphascreen^® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN₃) or metal ions (Fe²⁺, Fe³⁺, Cu²⁺, Zn²⁺ and Ni²⁺). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen^® assays.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only