PRMT1 Chemiluminescent Assay Kit

Catalog #

52004L

$790 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The PRMT1 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT1 activity for screening and profiling applications. The key to the PRMT1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against PRMT1? Check out our Methyltransferase Screening Services.

This product has been cited 8 times.

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Synonyms

PRMT1, HRMT1L2, IR1B4, HCP1, PRMT-1

Product Info

Storage and Usage

Citations8

Assay Kit Format

Chemiluminescent

Supplied As

Kit comes in a convenient format, with 8-well strips precoated with histone H4 peptide substrate, reagents, and purified PRMT1 enzyme for 96 enzyme reactions.

Format

Catalog Number	Component	Amount	Storage
51040	PRMT1 human recombinant enzyme	10 µg	-80°C	*Avoid freeze/ thaw cycles!*
52120	20 µM S-adenosylmethionine*	250 µl	-80°C
52150	Primary antibody 4	100 µl	-80°C
52131H	Secondary HRP-labeled antibody 2	10 µl	-80°C
52170	4x HMT assay buffer 2	3 ml	-20°C
52100	Blocking buffer 4	50 ml	+4°C
79670	ELISA ECL Substrate A (transparent bottle)	6 ml	RT
79670	ELISA ECL Substrate B (brown bottle)	6 ml	RT
	96-well plate precoated with histone substrate	1 plate	+4°C

UniProt #

Q99873

Background

PRMT1 is a histone methyltransferase that exhibits methylation activity toward H4-R3.

References

Dillon S.C., et al. 2005. Genome Biology 6:227.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Notes

* Decreasing S-adenosylmethionine concentration will make the assay more sensitive to the inhibitors.

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only