NSD2 Chemiluminescent Assay Kit
The NSD2 Direct Activity Chemiluminescent Assay Kit is designed to measure NSD2 activity for screening and profiling applications. The key to the NSD2 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S- adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
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TBST buffer (1 x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform
Catalog Number |
Component |
Amount |
Storage |
|
51026 | NSD2 human enzyme | 50 µg | -80°C | Avoid freeze/ thaw cycles! |
52120 | 100 μM S-adenosylmethionine | 250 µl | -80°C | |
52140P2 | Primary antibody 16-2 | 12.5 µl | -80°C | |
52131H | Secondary HRP-labeled antibody 2 | 10 µl | -80°C | |
52139Z | 4x HMT assay buffer 7 | 3 ml | -20°C | |
79556 | Blocking buffer 1 | 50 ml | +4°C | |
HRP chemiluminescent substrate A (translucent bottle) |
6 ml |
+4°C |
||
HRP chemiluminescent substrate B (brown bottle) |
6 ml |
+4°C |
||
White microplate precoated with histone substrate |
1 |
+4°C |