NSD2 Chemiluminescent Assay Kit

Catalog #
53009
$750 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The NSD2 Direct Activity Chemiluminescent Assay Kit is designed to measure NSD2 activity for screening and profiling applications. The key to the NSD2 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S- adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against NSD2? Check out our Methyltransferase Screening Services

Synonyms
WHSC1, Wolf-Hirschhorn Syndrome Candidate 1, MMSET, Multiple Myeloma SET Domain Containing Protein, NSD2, Nuclear SET Domain-Containing Protein 2, NSD
Product Info
Storage and Usage
Citations
Assay Kit Format
Chemiluminescent
Supplied As
Kit comes in a convenient format, with 96-well plate precoated with histone H3 substrate, reagents, and purified NSD2 enzyme for 96 enzyme reactions.
Materials Required But Not Supplied

TBST buffer (1 x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format
Catalog
Number

Component

Amount

Storage
51026 NSD2 human enzyme 50 µg -80°C


Avoid freeze/
thaw cycles!
52120 100 μM S-adenosylmethionine 250 µl -80°C
52140P2 Primary antibody 16-2 12.5 µl -80°C
52131H Secondary HRP-labeled antibody 2 10 µl -80°C
52139Z 4x HMT assay buffer 7 3 ml -20°C
79556 Blocking buffer 1 50 ml +4°C
HRP chemiluminescent substrate A
(translucent bottle)

6 ml

+4°C
HRP chemiluminescent substrate B
(brown bottle)

6 ml

+4°C
White microplate precoated with
histone substrate

1

+4°C
UniProt #
O96028
References
Dillon S.C., et al. 2005. Genome Biology 6:227.