MLL1 Complex Chemiluminescent Assay Kit

Catalog #
53008
$750 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The MLL1 Complex Direct Activity Chemiluminescent Assay kit is designed to measure MLL1 activity for screening and profiling applications, using purified MLL1 and its complex components: WDR5, RbBP5,Ash2, and DPY30. The key to the MLL1 Complex Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K4 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

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This product has been cited 1 time.

Synonyms
KMT2A, MLL1A, CXXC7, ALL-1
Product Info
Storage and Usage
Citations1
Assay Kit Format
Chemiluminescent
Supplied As
Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, reagents, and purified MLL1 enzyme complex for 100 enzyme reactions.
Materials Required But Not Supplied
  • TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween-20)
  • Luminometer or microplate reader capable of reading chemiluminescence
  • Adjustable micropipettor and sterile tips
  • Rotating or rocker platform
  • 0.5 M DTT
Format
Catalog Number
Component

Amount

Storage
51021 MLL1/WDR5/Ash2L/RbBP5/DPY30 25 µg -80°C 





Avoid

freeze/ thaw cycles! 
52120 20 µM S-adenosylmethionine  250 µl -80°C 
52140Z Primary antibody 26 12.5 µl -80°C 
52160 4x HMT assay buffer 1* 3 ml -20°C
52131H Secondary HRP-labeled antibody 2 10 µl -80°C 
52100 Blocking buffer 4 50 ml +4°C 
79670 ELISA ECL substrate 
(2 components)
6 ml each Room Temp
  96-well plate pre-coated with histone substrate  1 plate +4°C 
























*Add 125 µl of 0.5 M DTT before use. 

UniProt #
Q03164 (MLL1), P61964 (WDR5), Q9UBL3 (Ash2L), Q15291 (RbBP5), Q9C005 (DPY30)
References

Dillon SC, Zhang X, Trievel RC, Cheng X. Genome Biology 2005; 6:227.