EZH2 (A738T) Chemiluminescent Assay Kit
The EZH2 (A738T) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (A738T)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes. The key to the EZH2 (A738T) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
TBST buffer (1x Tris-buffered saline, pH 8.0, containing 0.05% Tween-20)
Luminometer or microplate reader capable of reading chemiluminescence
Rotating or rocker platform
| Catalog Number |
Component | Amount | Storage | |
| 51061 | EZH2 (A738T)/EED/SUZ12/ RbAp48/AEBP2 |
5 μg | -80°C | Avoid freeze/ thaw cycles! |
| 52120 | 20 μM S-adenosylmethionine | 3 x 250 μl | -80°C | |
| 52140F | Primary Antibody 6 | 12.5 μl | -80°C | |
| 52131H | Secondary HRP-labeled Antibody 2 | 10 μl | -80°C | |
| 52170 | 4x HMT Assay Buffer 2 | 3 ml | -20°C | |
| 79556 | Blocking Buffer 1 | 50 ml | +4°C | |
| 79670 | ELISA ECL substrate A (translucent bottle) |
6 ml | RT | |
| ELISA ECL substrate B (brown bottle) |
6 ml | RT | ||
| 96-well plate precoated with histone substrate | 1 plate | +4°C | ||
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