EZH1 Chemiluminescent Assay Kit
The EZH1 Chemiluminescent Assay Kit is designed to measure activity of the EZH1 complex (EZH1/EED/SUZ12/RbAp48/AEBP) for screening and profiling purposes. The key to the EZH1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRPlabeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Need us to run inhibitor screens or profile your compounds against EZH1? Check out our Methyltransferase Screening Services.
TBST buffer (1x TBS, pH 8.0, containing 0.05% Tween-20)
Luminometer or microplate reader capable of reading chemiluminescence
Rotating or rocker platform
| Catalog Number |
Component |
Amount |
Storage |
|
| 51007 | EZH1/EED/SUZ12/RbAp48/AEBP2 | 25 µg | -80°C | Avoid freeze/ thaw cycles! |
| 52120 | 400 μM S-adenosylmethionine | 250 µl | -80°C | |
| 52140F | Primary antibody 6 | 25 µl | -80°C | |
| 52131H | Secondary HRP-labeled antibody 2 | 10 µl | -80°C | |
| 52170 | 4x HMT assay buffer 2 | 3 ml | -20°C | |
| 79556 | Blocking buffer 1 | 50 ml | +4°C | |
79670 |
ELISA ECL substrate A (translucent bottle) |
6 ml |
Room Temp. |
|
| ELISA ECL substrate B (brown bottle) |
6 ml |
Room Temp |
||
| 96-well plate precoated with histone substrate |
1 plate |
+4°C |
||
2. Morin, RD., et al. Nat. Genet. 2010, 42(2):181.
Powered by Bioz
San Diego, CA 92121
858-202-1401
Products are for Research Use Only
COMPANY
Copyright © 2024
BPS Bioscience, Inc.
All rights reserved.
Diagrams on this website are created with BioRender.com.