SETD2 Chemiluminescent Assay Kit
Catalog # 52060
Size: 96 reactions
DESCRIPTION: The SETD2 Chemiluminescent Assay Kit is designed to measure SETD2
activity for screening and profiling applications. The SETD2 Chemiluminescent Assay Kit
comes in a convenient strip format, with a 96-well plate precoated with histone H3 peptide
substrate, the antibody against methylated lysine36 residue of Histone H3, the secondary
HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and
purified SETD2 enzyme for 100 enzyme reactions. The key to the SETD2
Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K36
residue of Histone H3. With this kit, only three simple steps on a microtiter plate are
required for methyltransferase detection. First, S-adenosylmethionine is incubated with a
sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary
antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody
followed by addition of the HRP substrate to produce chemiluminescence that can then be
measured using a chemiluminescence reader.
APPLICATIONS: Great for studying enzyme kinetics and HTS applications.
STABILITY: One year from date of receipt when stored as directed.
REFERENCE: Dillon SC, Zhang X, Trievel RC, Cheng X. Genome Biology 2005; 6:227.