EHMT1 (GLP) Chemiluminescent Assay Kit
Catalog # 53007

DESCRIPTION: The EHMT1 (GLP) Chemiluminescent Assay Kit is designed to measure
EHMT1 (also known as G9a-like protein) activity for screening and profiling applications.
The EHMT1 (GLP) Chemiluminescent Assay Kit comes in a convenient format, with a 96-
well strip plate precoated with histone H3 peptide substrate, primary antibody against
methylated lysine residue of Histone H3, the secondary HRP-labeled antibody, Sadenosylmethionine,
methyltransferase assay buffer, and purified EHMT1 for 100 enzyme
reactions. The key to the EHMT1 (GLP) Chemiluminescent Assay Kit is a highly specific
antibody that recognizes methylated K9 residue of H3.
With this kit, only three simple steps on a microtiter plate are required for
methyltransferase detection. First, S-adenosylmethionine is incubated with a sample
containing assay buffer and methyltransferase enzyme for one hour. Next, primary
antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody
followed by addition of the HRP substrate to produce chemiluminescence that can then be
measured using a chemiluminescence reader. Note: The kit is also suitable for use with
cell extracts, but non-specific methyltransferase activity may be detected.

COMPONENTS:

APPLICATIONS: Great for studying enzyme kinetics and HTS applications.

STABILITY: One year from date of receipt when stored as directed.

REFERENCE: Huang, J., et al., J Biol. Chem. 2010 Mar 26; 285(13): 9636-41.

Click here to view and download protocol (PDF)