The L3MBTL1 Inhibitor Screening Assay Kit is designed to measure the inhibition of L3MBTL1 binding to its substrate. The key to the kit is the specific binding of the L3MBTL1 MBT domain to the methylated-peptide substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing L3MBTL1 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
Min, J., et al., Nat Struct Mol Biol.2008 Jan,15(1):114
Storage/Stability
Stable at least 6 months from date of receipt, when stored as directed
Instructions for Use
See data sheet for detailed protocol.
Applications
Useful for the study of MBT domain binding assays, screening inhibitors, and selectivity profiling
Shipping Temperature
-80°C
Notes
AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Contraindications
DMSO >1%, strong acids or bases, ionic detergents, high salt
Warnings
Avoid freeze/thaw cycles
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.